In vivo wound healing activity of a herbal ointment in rat

Document Type : Original paper

Authors

1 Neurophysiology Research Center, Shahed University, Tehran, Iran. Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

2 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.

3 Department of Pathology, School of Medicine, Shahed University, Tehran, Iran.

4 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.

Abstract

Background and objectives: The wounds are still the health tribulations at the present time. In the present research the effect of a new herbal ointment (Oppyheal) in treatment of rat’s wound has been investigated. The effectiveness of the ointment was compared with the Fibrinolysin/DNAse.
Methods: Fifty four male Wistar rats were used. The wound was created on the back cervical skin of the animals under anesthesia in three different sizes. The animals in each size group were randomly divided into three groups. The control group did not receive the ointments. The reference group was given the Fibrinolysin/DNAse and the third group was treated with the Oppyheal. The products were topically used once per day until the wounds of one group were completely healed. The size of the wound area was measured in days 0-20 by a standard reference ruler. The reduction in size of the wound was calculated and analyzed. The recovered skin of all animals were examined histologically, pResults: The results showed a significant difference in wound contraction between the treated groups and the control group (p<0.0001). The new skin of ointment-treated rats showed healing features in comparison to the control group.
Conclusion: This study may introduce a suitable topical ointment, Oppyheal, for wound care.

Keywords


[1] Enoch S, John Leaper D. Basic science of wound healing. Surgery. 2005; 23(2): 37-42.
[2] Pierce GF, Mustoe TA. Pharmacologic enhancement of wound healing. Annu Rev Med. 1995; 46: 467-481.
[3] Mekkes JR, Le Poole IC, Das PK, Kammeyer A, Westerhof W. In vitro tissue-digesting properties of krill enzymes compared with Fibrinolysin/DNAse, papain and placebo. Int J Biochem Cell Biol. 1997; 29(4): 703-706.
[4] Kavitha KV, Tiwari S, Purandare VB, Khedkar S, Bhosale S, Unnikrishnan AG. Choice of wound care in diabetic foot ulcer: A practical approach. World J Diabetes. 2014; 5(4): 546-556.
[5] Suguna L, Sivakumar P, Chandrakasan G. Effects of Centella asiatica extract on dermal wound healing in rats. Indian J Exp Biol. 1996; 34(12): 1208-1211.
[6] Davis RH, Donato JJ, Hartman GM, Haas RC. Anti-inflammatory and wound healing activity of a growth substance in Aloe vera. J Am Podiatr Med Assoc. 1994; 84(2): 77-81.
[7] Ezike AC, Akah PA, Okoli CO, Udegbunam S, Okwume N, Okeke C,  Iloani O. Medicinal plants used in wound care: a study of Prosopis Africana (Fabaceae) stem bark. Indian J Pharm Sci. 2010; 72(3): 334-339.
[8] Jia Y, Zhao G, Jia J. Preliminary evaluation: the effects of Aloe ferox Miller and Aloe arborescens Miller on wound healing. J Ethnopharmacol. 2008; 120(2): 181-189.
[9] Chithra P, Sajithla GB, Chandrakasan G. Influence of Aloe vera on the healing of dermal wounds in diabetic rats. J Ethnopharmacol. 1998; 59(3): 195-201.
[10] Öztürk N, Korkmaz S, Öztürk Y. Wound-healing activity of St. John's Wort (Hypericum perforatum L.) on chicken embryonic fibroblasts. J Ethnopharmacol. 2007; 111(1): 33-39.
[11] Mukherjeea PK, Verpoorteb R, Suresh B. Evaluation of in vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol. 2000; 70(3): 315-321.
[12] Süntar I, Akkol EK, Keleş H, Oktem A, Başer KHC, Yeşilada E. A novel wound healing ointment: a formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. J Ethnopharmacol. 2011; 134(1): 89-96.
[13] Wölfle U, Seelinger G, Schempp CM. Topical application of St. Johns Wort. Planta Med. 2014; 80(2-3): 109-120.
[14] Pushpangadan P, George V, Singh SP. Poppy.  In: Peter, KV, Ed. Handbook of herbs and spices. Handbook of herbs and spices. Cambridge: Woodhead Publishing Series limited, 2012.
[15] Hameed HA, Waris Nawab S. Avicenna [The Canon of Medicine]. New Delhi: Jamia Hamdard Printing Press, 1998.
[16] Hakim GM. Chishti ND. The traditional healer’s hand book, a classic guide to the medicine of Avicenna. Vermont: Healing Arts Press, 1991.
[17] Faridi P, Abolhassanzadeh Z, Zarshenas MM, Mohagheghzadeh A. Gout remedies in Traditional Iranian Medicine (TIM). Res Hist Med. 2012; 1(4): 105-145.
[18] Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Rep. 2005; 57(1): 90-96.
[19] Riahy S, Imany H, Khoshbaten A. Effect of topical application of phenytoin and honey in closure of open wound in male rats. Ann Mil Health Sci Res. 2009; 7(2): 73-79.
[20] Bozan B, Temelli F. Extraction of poppy seed oil using supercritical CO2. J Food Sci. 2003; 68(2): 422-426.
[21]  Ivorra MD, Payá M, Villar A. A review of natural products and plants as potential antidiabetic drugs. J Ethnopharmacol. 1989; 27(3): 243-275.
[22]  Mahomoodally MF. Traditional medicines in Africa: an appraisal of ten potent African medicinal plants. Evidence Based Complement Altern Med. 2013; Article ID 617459.  
[23]  Fox LT, du Plessis J, Gerber M, van Zyl S, Boneschans B, Hamman JH. In vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe malothii gel materials after single and multiple applications. Pharmacogn Mag. 2014; 10(2): S392-403.
[24] Anderson FA. Final report on the safety assessment of Aloe andongensis extract, Aloe andongensis leaf Juice, Aloe arborescens leaf extract, Aloe arborescens leaf juice, Aloe arborescens leaf protoplasts, Aloe barbadensis flower extract, Aloe barbadensis leaf, Aloe barbadensis leaf extract, Aloe barbadensis leaf juice, Aloe barbadensis leaf polysaccharides, Aloe barbadensis leaf water, Aloe ferox leaf extract, Aloe ferox leaf juice, and Aloe ferox leaf juice extract. Int J Toxicol. 2007; 26(2): 1-50.
[25]  El-Shemy HA, Aboul-Soud MA, Nassr-Allah AA, Aboul-Enein KM, Kabash A, Yagi A. Antitumor properties and modulation of antioxidant enzymes' activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction. Curr Med Chem. 2010; 17(2): 129-138.