- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101GC/Mass analysis of the volatile compounds of P. hyrcanicum diethyl ether extract and GC profiling of some Iranian Polygonum species373881ENS. SaeidniaMedicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.P. SarkhailPharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.F Moradi-AfrapoliFaculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.A.R. GohariMedicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, IranM. NikanMedicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.N. Mokhber-DezfuliMedicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.G.R. AminDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran,
Iran.A. HajiakhondiMedicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, TehranJournal Article20131225In this study, the relationship among four species of <em>Polygonum </em>(including <em>P. hyrcanicum </em>(three samples)<em>, P. persicaria, P. avicular, </em>and <em>P. hydropiper</em>) was investigated by GC profiling. Furthermore, the major compounds of the ethylic ether extract of <em>P. hyrcanicum</em> were identified by GC/MS as: α-bisabolol (17.5%), cedrol (15.9%), sesquisabinene hydrate (13.0%), α-elemol (10.5%) and trans-longipinocarveol (10.1%). All the identified compounds were sesquiterpenes and no monoterpene, fatty acid and/or hydrocarbone were detected in the extract. Chemical distances among the mentioned species were calculated in order to construct the dendrogram of closely related samples. Results indicated that the distance between two samples of <em>P. hyrcanicum</em> was considered to be short and their GC profiles were quite similar to each other and also there was a close relationship between the two samples of <em>Polygonum </em>with <em>P. avicular</em>. <em>P. hydropiper</em> was observed far from the two samples of <em>P. hyrcanicum</em> in comparison to other samples. Interestingly, <em>P. hyrcanicum, </em>gathered from Veresk, had no close relationship with other pairs of <em>P. hyrcanicum.</em>The results of this study support the phylogenetic relationships among these <em>Polygonum </em>species which was previously reported.https://www.rjpharmacognosy.ir/article_3881_c4bd14964f103d9f9af660c9f35107bd.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101Chitosan (middle-viscous) as an effective elicitor for silymarin production in Silybum marianum hairy root cultures9133902ENT. HasanlooDepartment of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box: 31535-1897, Karaj, Iran.S. EskandariDepartment of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box: 31535-1897, Karaj, Iran.
Department of Biology, Faculty of Science, Kharazmi University, Karaj, Iran.F. NajafiDepartment of Biology, Faculty of Science, Kharazmi University, Karaj, Iran.Journal Article20131225Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in <em>Silybum marianum</em> hairy root cultures. The root cultures established by infection with <em>Agrobacterium rhizogenes</em> AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to improve silymarin production. Total silymarin increased about 5.26-fold after 96 h of treatment with 30 mg/50 mL chitosan. Dry weight of the hairy roots reached the highest point (0.530 and 0.535 g) after 96 h in presence of 20 and 30 mg/50 mL chitosan, respectively. Five different flavonolignans were isolated; taxifolin, silychristin, silydianin, silybin and isosilybin) 0.133, 0.200, 0.120, 0.041 and 0.056 mg/g dry weight, respectively). 30 days old hairy roots were treated by 30 mg/50 mL chitosan in different times (12, 24, 48, 72, 96 and 120 h). The amount of silymarin accumulation significantly increased (0.705 mg/gDW) in hairy roots after 96 h treatment. These observations suggested that the medium molecular weight of chitosan could be elicited by <em>S. marianum</em> hairy root cultures that lead to the higher production of silymarin. These results correlated with the culture time, and the biosynthesis which reached to a maximum of 0.705 mg/gDW by 96 h after culture. (2.9-fold higher than the control).https://www.rjpharmacognosy.ir/article_3902_400f8a26b0320a137aeaecc0e0554546.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101Anti-inflammatory effect, total polysaccharide, total phenolics content and antioxidant activity of the aqueous extract of three basidiomycetes15213903ENM. VazirianDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.S. DianatDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.A. ManayiDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.R. ZiariDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.A. MousazadehAgriculture and Natural Research Center of Mazandaran, Passand Forest and Rangeland Research Station, P.O. Box 167, Behshahr, Iran.E. HabibiDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.S. SaeidniaMedicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.Y. AmanzadehDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Journal Article20131225Inflammation is a part of the non-specific immune response which occurs in reaction to any type of injury. Medicinal mushrooms have had application in various disorders including cancer, liver injuries, inflammation and diabetes. In the present study, the anti-inflammatory effects of the aqueous extracts of medicinal mushrooms (<em>Fomes fomentarius</em>, <em>Ganoderma applanatum </em>and <em>Trametes hirsuta</em>) were evaluated using carrageenan method. In addition, total polysaccharide, total phenolics contents and the radical scavenging activity of the extracts have also been examined. Mushrooms were extracted with distilled water in 100 °C for 4 hours and then the extracts were freeze dried. Indomethacin was considered as the positive control in the anti-inflammatory evaluation. Polysaccharide contents of <em>F. fomentarius</em>, <em>G. applanatum</em>, and <em>T. hirsuta</em> extracts were assessed as 53.3±0.2, 31.7±0.03, and 19.1±0.6 glucose equivalent µg/100 µgEXT and total phenolic contents of them were successfully revealed as 9.9±0.2, 8.2±0.1, and 8.8±0.2 µgGAE/100 µgEXT, respectively. Furthermore, the IC<sub>50</sub> values for <em>F. fomentarius</em>, <em>G. applanatum</em>, and <em>T. hirsuta</em> extracts in DPPH assay, were calculated as 90.9, 108.6, and 908.3 µg/mL, respectively. The results of the experiment showed that the extracts possessed potent anti-inflammatory effect which was comparable to indomethacin.https://www.rjpharmacognosy.ir/article_3903_00ee741f034e020c23c16ce75e000da3.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine23263905ENS.E. Sadat-EbrahimiDepartment of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.M. Hassanpoor MirPharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.G.R. AminDepartment of pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.H. HajimehdipoorDepartment of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Journal Article20131225<em>Securigera securidaca </em>(L.) Degen & Dorfl grows in different parts of Iran. The seeds of the species are used in Iranian folk medicine as an anti-diabetic agent. Many studies have established hypoglycemic effects of amino acids and in the present investigation, amino acids of <em>Securigera securidaca </em>seeds have been evaluated. The ground seeds were extracted using petroleum ether, hot ethanol and ethanol 50%, respectively. ethanol 50% extract was chromatographed over cation exchanging resin and the resulting amino acid fraction was subjected to HPLC after OPA derivatization and the amino acids were identified by comparing to standards. The results evidenced the presence of 19 amino acids in the plant extract including alanine, arginine, asparagine, aspartic acid, citrulline, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine and valine. Considering the role of some amino acids in diabetes the above amino acids could be noted as hypoglycemic agents of the plant seeds but further studies are necessary.https://www.rjpharmacognosy.ir/article_3905_bbac27d5aca927737db112abc9190b3a.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101A survey of cytotoxic effects of some marine algae in the Chabahar coast of Oman Sea27313906ENM. MosaddeghTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.B.M. GharanjikOffshore Waters Research Center, Chabahar, Iran.F. NaghibiTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.S. EsmaeiliTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.A. PiraniTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.B. Eslami TehraniTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.B. KeramatianTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.A. HassanpourTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Journal Article20131225Iran has 1260 km of coastline that borders the Persian Gulf and the Oman Sea in the northwest Indian Ocean. Marine algae are one of the natural resources in the marine ecosystem which produce a wide range of new secondary metabolites with various biological activities that play an important role in the pharmaceutical care. In this study the cytotoxic activity of 28 marine algae of Chabahar coast was assessed against 5 cell lines including MCF-7, HepG-2, A-549, HT-29 and MDBK, through MTT assay. The methanol extract of the algae did not show cytotoxicity against any of the tested cell lines up to 100 μg/mL concentration, except for <em>Jania adhaerens </em>(IC<sub>50</sub> 85.03 µg/mL) against MCF-7 cells.https://www.rjpharmacognosy.ir/article_3906_334f81899804ec818cbd3c82197f71ab.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101Evaluating the antioxidant and acetylcholinesterase inhibitory activity of three Centaurea species33373908ENH. HajimehdipoorTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.F. NaghibiTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.A. BandidarianTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.H. Moazzeni ZehanTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.A. PiraniTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.M. Hamzeloo-MoghadamDepartment of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Journal Article20131225Factors such as oxidative stress and reduced acetylcholine level have been implicated in Alzheimer’s disease (AD) pathology and recently there has been a trend towards natural product research to find potential sources of antioxidants and acetylcholinesterase inhibitors in the plants kingdom. <em>Centaurea</em> is a genus with about 500 species world wild, many of them have shown to possess biologic activity; <em>Centaurea albonites, C. aucheri and C. pseudoscabiosa </em>are three species which little investigation has been carried out about their biological properties. In the present study, the antioxidant and acetylcholinesterase inhibitory activity of the above mentioned species have been evaluated. The ability of the total extract and methanol fraction of the plants to scavenge free radicals has been assessed through DPPH radical scavenging assay, and the acetylcholinesterase inhibitory property has been evaluated by Ellman method. The total extract of all species exhibited moderate antioxidant activity whereas the extracts of <em>C. pseudoscabiosa </em>showed the strongest antioxidant property; its total extract also demonstrated the highest acetylcholinesterase inhibitory activity among the evaluated samples (19.2% inhibition). The results suggest the species as potential sources of natural antioxidants which could be focused in future studies of Alzheimer’s disease.https://www.rjpharmacognosy.ir/article_3908_260742439cd98f8e5c5014fb5055f2d2.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101In vitro antifungal activity of four medicinal plants used in Iranian Traditional Medicine39433911ENS.J. HashemiDepartment of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.J. AsgarpanahDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad
University (IAU), Tehran, Iran.Z. AlaeeDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad
University (IAU), Tehran, Iran.S. SadeghianDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad
University (IAU), Tehran, Iran.H. HasaniDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad
University (IAU), Tehran, Iran.A. AzimiDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad
University (IAU), Tehran, Iran.Journal Article20131225Evaluating the <em>in vitro </em>antifungal activity of <em>Phlomis lanceolata, Rhynchocorys elephas, Otostegia persica </em>and <em>Eremurus persicus</em>, four species used in Iranian Traditional Medicine, has been performed on the clinical isolates of the pathogenic fungi <em>Aspergillus fumigatus, A. flavus, Trichophyton mentagrophytes, T. rubrum, T. verrucosum, Microsporum canis, M. gypseum and Epidermophyton floccosum</em> and the yeast <em>Candida albicans</em>. The susceptibility tests were done by agar disc diffusion method and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of active extracts and sub-fractions were measured using the method of National Committee for Clinical Laboratory Standards (NCCLS). Among the investigated species,<em> P. lanceolata</em> sub-fractions were found to have fungicidal activity. The MIC and MFC was found to be considerable in petroleum ether, chloroform and ethyl acetate fractions (100 and 200 mg/mL) against the studied fungi and the yeast <em>Candida albicans</em>. The species appears to be a promising remedy for fungal based diseases, yet further studies are necessary.https://www.rjpharmacognosy.ir/article_3911_c8d70f8a57215c2b4ae19b5c0f8cff70.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44581120140101Evaluation of antioxidant activity of Ruta graveolens L. extract on inhibition of lipid peroxidation and DPPH radicals and the effects of some external factors on plant extract's potency.45503912ENS. Mohammadi- MotamedDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.S. Shahidi-MotlaghDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.H. BagherzadehDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.S. Azad ForouzDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.H. TafazoliDepartment of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.Journal Article20131225The antioxidant properties of <em>Ruta graveolens </em>L. were evaluated by two different methods; free radical scavenging using DPPH and inhibition of lipid peroxidation by the ferric thiocyanate method. The IC<sub>50</sub> value of the methanol extract in DPPH inhibition was 200.5 μg/mL which was acceptable in comparison with BHT (41.8 μg/mL). In thiocyanate method, the plant extract demonstrated activity as much as BHT in prevention of lipid peroxidation. Increasing the temperature during extraction, significantly decreased the extract power in inhibition of DPPH radicals. The storage time and temperature had no effect on lipid peroxidation inhibition.https://www.rjpharmacognosy.ir/article_3912_44451e36fc0fd4fd8de17c049a866a7f.pdf