eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
1
5
6326
Isolation and characterization of phytochemicals of Johrenia paucijuga (DC.) Bornm.
A.R. Gohari
1
S. Saeidnia
2
A. Naseri
3
A. Manayi
4
I. Salimikia
5
H.R. Monsef Esfahani
6
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Background and objectives: The genus Johrenia belongs to Umbelliferae family and contains five species that are endemic to Iran. Johrenia paucijuga grows widely in the north-west, west and center of Iran. So far, there has been no research about phytochemistry of J. paucijuga. In the present study, phytochemicals of the plants have been isolated and their structures have been elucidated. Methods: The aerial parts were dried and cut into small pieces, then extracted with ethyl acetate and methanol using perculator apparatus at room temperature. The methanol extract was extracted again with, petroleum ether and butanol. The separation and isolation process was carried out using column (silica gel and Sephadex LH-20) and thin layer chromatographic (TLC) methods. Structure elucidation of the purified compounds were based on ¹H and ¹³C-NMR data, in comparison with those reported in the previous literatures. Results: The isolated compounds from the ethyl acetate and butanol extracts of J. paucijuga were identified as β-sitosterol, β- stigmasterol and quercetin. Quercetin is a bioactive flavonoid widely used as a health supplement. β- sitosterol and β-stigmasterol are phytosteroles (plants sterols) with chemical structure similar to that of cholesterol and are sometimes used in treating hypercholesterolemia. Conclusion: Regarding the valuable biological properties of the isolated compounds, different biological effects could be expected from the plant.
https://www.rjpharmacognosy.ir/article_6326_ded0ba05e9fcedc0bc23b37728c8d98f.pdf
β-sitosterol
β-stigmasterol
Johrenia paucijuga
phytochemistry
Quercetin
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
7
10
6330
A survey about prophage induction ability in Escherichia coli K-12(λ) by ethnic medicinal plants of Kohgiluyeh va Boyerahmad, Iran
M. Hamzeloo-Moghadam
1
B. Eslami Tehrani
2
A. Pirani
3
A. Hassanpour
4
S. Esmaeili
5
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background and objectives: There is a growing trend towards investigating natural products as sources of compounds with biological effects and many researches have been carried out in order to find effective medications against many diseases. Cancer is no exception and studies focusing on evaluating the effects of different materials on DNA, give valuable information in cancer researches and carcinogenicity studies; thus the present study was focused on evaluating the impact of medicinal plants from Kohgiluyeh va Boyerahmad province, Iran on DNA. Methods: Thirty five plant species collected have been investigated for prophage induction ability in Escherichia coli K-12(λ)throughinductest. Results:The assay demonstrated that 8 plants were able to affect DNA. Conclusion: The results confirm the role of natural resources for biologic effects and what’s more, potential drug candidates in new drug discovery.
https://www.rjpharmacognosy.ir/article_6330_1c094d23873862055afd1f9ef7973465.pdf
Escherichia coli K-12(λ)
Ethnopharmacology
inductest
Kohgiluyeh va Boyerahmad
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
11
18
6331
Antioxidant and antibacterial activities of the essential oils and extracts of Dorema ammoniacum roots and aerial parts
M.R. Delnavazi
1
S. Tavakoli
2
A. Rustaie
3
H. Batooli
4
N. Yassa
5
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Isfahan Research Centre of Agriculture and Natural Sources, Kashan Station, Kashan, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Isfahan Research Centre of Agriculture and Natural Sources, Kashan Station, Kashan, Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Background and objectives: Dorema ammoniacum D. Don (Apiaceae) is a monocarpic plant endemic to central Iran. The gum resin of this species is a known therapeutic agent in Iranian, Indian and Western traditional medicines. The aim of the present study was to investigate essential oil constituents and evaluation of antioxidant and antibacterial activities of the essential oils and extracts of D. ammoniacum aerial parts and roots. Methods: Essential oils were analyzed using GC and GC/MS. The oils together with n-hexane, chloroform, ethyl acetate and methanol extracts of the plant samples were subjected to antioxidant evaluation by DPPH and FRAP assays and antibacterial screening using disk diffusion and micro-well dilution methods. Results: Thirty-four compounds were identified in the aerial parts oil, among them β-himachalene (9.3%) and β-chamigrene (8.7%) were the main constituents. Thirty-five compounds were also characterized in the roots oil, of which β-bisabolene (15.1%) and hexadecanal (13.2%) were the main components. Ethyl acetate extract of the roots showed the highest antioxidant activity in both DPPH (IC50 21.3 ± 2.7 μg/mL) and FRAP (112.7 ± 8.1 mmol FeSO4.7H2O equivalent/g) assays. In antibacterial assay, the ethyl acetate and chloroform extracts of the roots exhibited strong antibacterial activity against Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Essential oils were also found to be active against Shigella dysenteriae. Conclusion: Considerable antioxidant and antibacterial activities of D. ammoniacum make it an appropriate candidate for further studies and identification of its bioactive principles.
https://www.rjpharmacognosy.ir/article_6331_1e009c44262c92e7c52d10390a1633fe.pdf
antibacterial
Antioxidant
Apiaceae
Essential oil
Dorema ammoniacum
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
19
25
6333
Cytotoxic activity screening of some medicinal plants from south of Iran
S. Esmaeili
1
M. Hamzeloo-Moghadam
2
S. Ghaffari
3
M. Mosaddegh
4
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background and objectives: Cancer is a public health problem all over the world. Herbal medicines have a vital role in the prevention and treatment of cancer and large numbers of plants and their isolated constituents have shown potential anticancer activity. Methods: Twenty seven medicinal plants from southern Iran provinces have been extracted with methanol and screened for their cytotoxic activity against MCF-7, WEHI-164, HepG-2, MDBK and A-549 cell lines by MTT assay. Results: The methanol extracts of two species, Calotropis procera andJuniperus excelsa, demonstrated to be more effective compared to other extracts. Conclusion: The above species are proper candidates for further cancer studies.
https://www.rjpharmacognosy.ir/article_6333_e5fd5675d3d9f0e8d1699d7c329172d3.pdf
Cytotoxic activity
Iran
medicinal plants
MTT assay
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
27
33
6334
Chemical composition and leishmanicidal activity of Pulicaria gnaphalodes essential oil
G. Asghari
1
F. Zahabi
2
A. Eskandarian
3
H. Yousefi
4
M. Asghari
5
Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Parasitology, faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Parasitology, faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Biotechnology, College of Agriculture & Natural Resources,University of Tehran, Tehran, Iran.
Background and objectives: Several natural compounds have been identified for the treatment ofleishmaniasis. Due to a few safe drugs and the side effects caused by available chemotherapy, some new drugs for treatment of leishmaniasis are requested. The genus Pulicaria (Asteraceae) is represented in the flora of Iran by five species. Phytochemical studies on Pulicaria species have revealed some flavonoids and terpenoids with leishmanicidal activity. In the present investigation chemical composition and leishmanicidal activity of Pulicaria gnaphalodes essential oil have been studied. Methods: The essential oil of the aerial parts of the plant was obtained by Clevenger apparatus and was analyzed by GC/MS. Antileishmanil activity was assessed against promastigoes of Leishmania major. Results:The major components from P. gnaphalodes essential oil have been reported to be geraniol, 1,8-cineole, chrysanthenone, α-pinene, chrystanthenone, α-terpineol and filifolone. The alcohol monoterpenes with contribution of 25.04% constituted the major portion of the essential oil, while hydrocarbon monoterpenes and hydrocarbon sesquiterpenes with contribution of 7.08% and 2.38%, respectively occupied the next rates.In the present experiment the essential oil of P. gnaphalodes progressively inhibited Leishmania major growth in concentrations ranging from 0.125 to 50 µL/mL (parasite culture) in 24 h. The essential oil at 50 µL/mL eliminated the promastigotes at the beginning of treatment. It showed antileishmanial activity in concentration of 1.06 µL/mL and destroyed all parasits in 24 h. Conclusion: Pulicaria gnaphalodes antileishmanial activity, could suggest the species and constituents as possible lead structures for antileishmanial drug discovery.
https://www.rjpharmacognosy.ir/article_6334_0a4481ff29487ef32351aa2e0f1af5d0.pdf
Essential oil
GC/MS
Leishmania Major
Pulicaria gnaphalodes
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
35
39
6336
Antibacterial activity of ethanol extract and fractions obtained from Taraxacum mongolicum flower
H. Qiao
1
T.J. Sun
2
School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, PR China
School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, PR China
Background and objectives: Resistance towards reveling antibiotics has captured great interest in evaluating the antimicrobial properties of the natural plants. Taraxacum mongolicum is widely used as a folklore medicinal plant for its diuretic, antirheumatic and anti-inflammatory properties. Though there are some reports on antimicrobial properties of Taraxacum mongolicum, studies on antibacterial abilities of its flower are limited and it was decided to evaluate the antibacterial properties of the flowers in the present study. Methods: The antibacterial properties of ethanol extract of Taraxacum mongolicum flower, and its fractions (petroleum ether, ethyl acetate (ET), and aqueous fractions) were examined through agar disc diffusion method, and the minimum inhibitory concentration (MIC) was determined. Four Gram-negative and two Gram-positive bacteria were used in the study. Results: The antibacterial test results showed that the ET fraction strongly inhibited the growth of all of the microorganisms, especially Pseudomonas aeruginosa and Bacillus subtilis (with MIC values of 125 μg/mL and 62.5 μg/mL, respectively), whereas the ethanol extract and the other two fractions demonstrated moderate and weak activities, respectively. Conclusion: The ET fraction obtained from Taraxacum mongolicum flowers presented high antibacterial activity and might be suggested for use as a natural preservative ingredient in pharmaceutical industries.
https://www.rjpharmacognosy.ir/article_6336_2ced70c144dd6d92ce68e5239bf207d7.pdf
Antibacterial Activity
Minimum inhibitory concentration
Taraxacum mongolicum
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
41
47
6337
Seasonal variation of mono- and sesquiterpenoid components in the essential oil of Dracocephalum kotschyi Boiss.
G. Asghari
1
N. Keyhanfard
2
Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Background and objectives: Dracocephalum kotschyi is a plant which belongs to the Lamiaceae family and exists mostly in south-west Asian countries, including Iran. This plant is used as antispasmodic, analgesic and anti-inflammatory to treat rheumatoid diseases. Methods: In order to investigate the impact of the harvesting time changes on the quantity and quality of mono- and sesquiterpenoid components of D. kotschyi aerial parts, ten samples were collected from cultivated plants from 19 April to 27 August 2013. Also samples of flower and root were harvested in order to investigate their essential oil components. The essential oils were obtained through hydrodistillation method. The components were studied and identified by GC and GC ⁄ MS systems. Results: The highest yield of the essential oil was obtained on 3 May (1.10% V.W) and the lowest on 28 July (0.29% V.W). Totally 55 compounds were identified in the essential oil while the highest percentage belonged to monoterpenes especially the oxygenated ones. Most variations were observed in geraniol (1.40-15.34%), geranyl acetate (trace-14.41%) and neryl acetate (0.62-17.51%). The major value in most cases belonged to geranial. Conclusion: the results of this study indicate that the harvesting time of plant is an effective factor in the quality and quantity of theessential oil of Dracocephalum kotschyi.
https://www.rjpharmacognosy.ir/article_6337_bd0a61f3081d19e32010db4a30087d83.pdf
Dracocephalum kotschyi
Essential oil
geranial
Lamiaceae
Seasonal variations
eng
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
2014-10-01
1
4
49
55
6339
Role of micronutrients and natural antioxidants in fighting against HIV; a quick mini-review
S. Saeidnia
1
M. Abdollahi
2
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Canada.
Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
Oxidative stress has been implicated in the progression of HIV to AIDS, since HIV usually replicates in a highly oxidized condition and CD4+ T lymphocytes can be activated via a cascade of internal oxidative pathways, which enhances the formation of proteins and enzymes. Thus, antioxidants should potentially be useful for the treatment and prevention of HIV infection as a new alternative strategy. Regarding the point that there are various approaches for treating the HIV-positive patients, antioxidant supplementation therapy alongside with other medications possesses many benefits. In fact, antioxidants and micronutrient supplements have been considered as a costly and short-term strategy to improve antioxidant deficiency. If diets come with sufficient education and scientific recommendations, they can provide a low-cost and long-term strategy to reduce oxidative stress, prevent micronutrient deficiency, and slow down HIV progression. This strategy may be applicable and beneficial particularly in countries around coast of Africa, where HIV is most common. Meantime these countries are rich of natural food resources. It seems that a healthy diet is the best way to insure proper nutrient intake, since it contains many nutrients not available in pills.
https://www.rjpharmacognosy.ir/article_6339_8360fd8a9d8af1d4f62cfe459ff5a525.pdf
antioxidants
HIV
mechanism of action
phytochemicals